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KMID : 0358119930190020044
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Journal of the Korean Public Health Association
1993 Volume.19 No. 2 p.44 ~ p.54
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Transplacental Effects of Mutagenic Pesticides in Mice. -Induction of micronucleus and SCE in mouse fetal liver erythroblasts
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裵恩相/Bae, Eun Sang
金永煥/柳寅晟/Kim, Young Whan/Yu, In Sung
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Abstract
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This research was carried out to investigate the transplacental genotoxic effects of some mutagenic pesticides in mice. As for pesticides, we used pesticides such as organic phosphate insecticides (Dimethoate, Dichlorvos, Fenitrothion and Trichlorfon), carbamate insecticide (Carbofuran), phthalimide fungicids (Caplan, Captafol, Folpet) and herbicides (Machete, Pen tachlorophenol (pep)). These pesticides were injected intra peritoneum of mice on the 16th day of gestation with 12.5%, 25% and 50% of LD50 respectively.
Two fetuses per litter were extracted after twenty hours of injection, and micronucleated polychromatic erythrocyte (MNPCE) were observed in hepatic tissues, Six hours after injection, fetuses were extracted, and frequency of sister chromatid exchange (SCE) were measured after incubation of hepatic cells for 26 hours.
The results of the experiment were as follows
1. As for the Induction rate of MNPCE in fetal liver, 50% of LD50 treatment groups of Dichlorvos, Fenitrothion and Trichlorfon showed 3.0 to 4.8 times as high a rate as control (saline) group (p<0.05), Among 25% of LD50 treatment guorps, Fenitrothion only showed the higher rate than control group (pInduction rates of MNPCE in the Dimethoate group and Carbofuran group increased slightly, but it was not statistically significant. In the Captan, Captafol and Folpet treatment groups, all the groups which were treated by 50% of LD50 treatment, showed 2.0 to 4.6 times as high a rate as control (corn oil) group (p<0. 01), and all 25% of LD50 treatment groups showed 2.0 to 3.3 times higher rate (p<0.05).
Machete 50% and 25% of LD50 treatment groups showed 2.4 to 2.6 times as high a rate of
MNPCE as control (corn oil) group (p<0.05), PCP 50% of LD50 treatment group also showed increased induction rate of MNPCE.
2. As for the average SCE frequency of fetal liver erythroblast, all the 25% and 12.5% of
LD50 treatment groups showed 1.5 to 2.4 times as high as control (saline) groups except Dimethoate.
Especially Captafol treatment group showed increased SCE frequency apparently (p
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